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[Determination of puerarin in xinmaitong oral liquid by HPLC]
1Third Affiliated Hospital, Sun Yat-sen University of Medical Sciences, Guangzhou 510630, Guangdong, China.
Objective:
To develop a method to determine the content of puerarin in Xinmaitong oral liquid.
Methods:
HPLC method was used. The separation was performed on Suntek kromasil C18 column with methanol-water containing 0.5% acetic acid (25:75) as a mobile phase and the wavelength of UV detector was 250 nm.
Results:
The linearity of puerarin was good and average recovery was 97.8%.
Conclusion:
The method is simple, reliable and sensitive. It also shows good resolation. It can be used in quality control of Xinmaitong oral liquid.
