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Updated: Aug 15, 2026
Analytical Techniques for Assaying Nitric Oxide Bioactivity
Published on: June 18, 2012
[Inhibitory effect of artemin on endotoxin-induced nitric oxide synthesis]
1Institute of Chinese Materia Medica, China Academy of Traditional Chinese Medicine, Beijing 100700, China.
Objective:
To investigate the effect of Artemin on LPS-induced nitric oxide synthesis in macrophages.
Method:
1. Nitrite oxide (NO) production of RAW 264.7 cells was induced by LPS or LPS in combination with interferon-gamma (IFN gamma) in the presence or absence of Artemin. The amount of NO in the supernatant of RAW 264.7 cells was detected with Griess reagent. 2. Balb/c mice were injected with Artemin (i.m.) 50 mg.kg-1.d-1 x 3 d, and intraperitoneal macrophages were collected to detect the LPS-induced NO production.
Result:
LPS 1.0, 0.2 microgram.ml-1 or IFN-gamma 100u + LPS 1.0, 0.2, 0.04 microgram.ml-1 could induced a large amount of NO synthesis of RAW 264.7 cells. Artemin showed a significant inhibitory effect on LPS or IFN-gamma + LPS-induced NO production in a dose dependent manner. After treatment with Artemin, the response of Balb/c mice to LPS was reduced, which was showed by a decrease in NO production of intraperitoneal macrophages induced by LPS.
Conclusion:
Artemin could reduce LPS-induced production of inflammatory factors resulting in the inhibition of inflammatory effects.
