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Updated: Aug 7, 2026
A Flow Cytometry-based Assay for Measuring Mitochondrial Membrane Potential in Cardiac Myocytes After Hypoxia/Reoxygenation
Published on: July 13, 2018
[The protective effects of yi-xin-kang capsule on the structure and function of rat myocardial mitochondria]
Object:
To observe the injury induced by Isoproterenol (ISO) on the structure and function of rat myocardial mitochondria and the protective effects of Yi-Xin-Kang Capsule (H303).
Method:
After injection of ISO, rat myocardial mitochondria were isolated. The activities of phospholipidase A2(PLA2) and Ca(2+)-ATPase, the content of phospholipids (PL) and high-energy phosphates, membrane lipid fluidity (LFU) and respiration function were determined respectively.
Result:
ISO induced severe injury to myocardial mitochondria. The activity of PLA2 was significantly activated. The intensity of LFU, the activity of Ca(2+)-ATPase, respiration function of mitochondria and the contents of PL and high-energy phosphates of myocardium tissue were significantly decreased. Oral administration of H303 significantly decreased the activity of PLA2. The contents of PL and LFU, the activity of Ca(2+)-ATPase were significantly increased. The respiration function of mitochondria was protected by accelerating III state respiration rate, improving ADP/O ratio and recovering the depressed respiration control rate.
Conclusion:
H303 possessed protective effect on structural and functional injury of mitochondria induced by ISO.
Insights
Yi-Xin-Kang Capsule (H303) protects rat heart mitochondria from Isoproterenol (ISO)-induced injury. H303 reversed structural and functional damage, improving mitochondrial respiration and energy metabolism.
Area of Science:
- Cardiovascular Research
- Mitochondrial Biology
- Pharmacology
Background:
- Isoproterenol (ISO) induces myocardial mitochondrial injury.
- Mitochondrial dysfunction is central to cardiac damage.
Purpose of the Study:
- To investigate ISO-induced injury on rat myocardial mitochondria.
- To evaluate the protective effects of Yi-Xin-Kang Capsule (H303).
Main Methods:
- Isolated rat myocardial mitochondria post-ISO injection.
- Assessed phospholipidase A2 (PLA2), Ca(2+)-ATPase activity, phospholipid (PL) and high-energy phosphate content, membrane lipid fluidity (LFU), and respiration function.
Main Results:
- ISO significantly activated PLA2 and decreased LFU, Ca(2+)-ATPase activity, mitochondrial respiration, PL, and high-energy phosphates.
- H303 administration reduced PLA2 activity and increased PL, LFU, and Ca(2+)-ATPase activity.
- H303 improved mitochondrial respiration by enhancing state III respiration, ADP/O ratio, and respiration control rate.
Conclusions:
- H303 demonstrates significant protective effects against ISO-induced myocardial mitochondrial structural and functional damage.
- H303 may serve as a therapeutic agent for conditions involving mitochondrial injury.
