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Updated: Sep 25, 2026
Assessment of DNA Contamination in RNA Samples Based on Ribosomal DNA
Published on: January 22, 2018
[Cluster analysis of Dendrobium by RAPD and design of specific primer for Dendrobium candidum]
1College of Life Sciences, Zhejiang University, Hangzhou 310012, Zhejiang, China.
Objective:
To analyze the genetic relationship of Dendrobium and design a specific primer to differentiate Dendrobium candidum effectively.
Method:
Random amplified polymorphic DNA (RAPD) technique was used to analyze the genetic relationships of 26 species of Dendrobium and one species of Ephemerantha. Dendrogram was constructed by UPGMA. According to the sequence of DNA fragment selected, Sangon 18 primer had been extended from 3' extreme to 20 bp in order to form a specific primer.
Result And Conclusion:
This primer can be used to distinguish Dendrobium candidum from other Dendrobium effectively. Such a technique provides a new way for the identification of Chinese traditional medicines.
