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[Research on the identification of penis et testis cervi with molecular taxonomy]
Shuang-yan Tang1, Wen Fu, Yong-jiu Chen
1Yunnan Provincial Institute for Drug Control, Kunming 650011, Yunnan, China.
Objective:
To make the kit with witch to identify Penis et Testis Cervi with molecular taxonomy.
Method:
The mtDNA of sika and red deer from different areas was amplified by PCR and sequenced. Compared with the mtDNA of bovine and horse from witch the false medicines were made, characteristic segments of deer were found. We selected one as the species distinctive PCR primer of deer.
Result:
The kit made up with this primer and related reagents could be used to discern Penis et Testis Cervi from the false medicine.
Conclusion:
It is a scientific, steady, accurate and convenient way to identify Penis et Testis Cervi with molecular taxonomy.