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[Determination of stilbene derivative in shengfa powders by HPLC]
Ben-quan Hu1, Ya-jun ShI, Ping Huang
1Department of Pharmacy, Tangdu Hospital, Fourth Military Medical University, Xi'an 710038, Shanxi, China. bqhu@21cn.com
Objective:
To develop a new method for the determination of the idiosyncratic component, 2,3,5,4'-tetrahydroxy-stilbene-2-O-beta-D-glycoside in Shengfa Powders.
Method:
A HPLC method was set up, using Hypersil BDS C18 column (5 microns, 4.6 mm x 150 mm), acetonitrile-water(18:82) as mobile phase, with detection at 320 nm.
Result:
The calibration curve was linear in the range of 0.45-2.25 micrograms, r = 0.9998, the average recovery was 99.5%, RSD = 1.2%(n = 6).
Conclusion:
The active constituent 2,3,5,4'-tetrahydroxy-stilbene-2-O-beta-D-glycoside in Shengfa Powders can be separated effectively. This method is simple, specific and exact.