Related Experiment Video
Updated: Sep 25, 2026

Preparation of Fungal and Plant Materials for Structural Elucidation Using Dynamic Nuclear Polarization Solid-State NMR
Published on: February 12, 2019
[Analysis of 1H-NMR fingerprint in stem of Dendrobium loddigesii]
Hai-lin Qin1, Jian-xin Zhang, Zheng-tao Wang
1Key Laboratory of Chemistry for Natural Products of Guizhou Province and Chinese Academy of Sciences, Guiyang 550002, Guizhou, China.
Objective:
To analyse the 1H-NMR finger-print of the stem of Dendrobium loddigesii.
Method:
Silica gel column chromatography was used to separate the chemical constituents of SCE A of the stem of D. loddigesii. The characteristic signals of the H-NMR finger-print were analysed after determining the structures of the compounds isolated from SCE A.
Result:
1H-NMR finger-prints of the samples of D. loddigesii collected from different regions showed highly characteristic features and reproducibility. Four compounds predominant in SCE A were isolated and their structures were determined by spectral analysis as 1, 2, 3 and 4, respectively.
Conclusion:
Compound 3 and 4 were isolated from D. loddigesii for the first time. The 1H-NMR finger-print of CGE A of the stem of D. loddigesii showed mainly the characteristic signals of the above four compounds and might be utilized for the original authentication of this plant.
Related Concept Videos
IR Frequency Region: Fingerprint Region
The...
¹H NMR: Interpreting Distorted and Overlapping Signals
As Δν decreases and the signals move closer, the doublets appear increasingly distorted. The intensities of the inner lines increase at the cost of those of the outer lines as the signals are slanted or...
NMR Spectroscopy of Aromatic Compounds
¹³C NMR: ¹H–¹³C Decoupling
A broadband decoupling technique is used to simplify these complex, sometimes overlapping, signals. Broadband decoupling relies on a...
¹H NMR of Conformationally Flexible Molecules: Variable-Temperature NMR
¹³C NMR: Distortionless Enhancement by Polarization Transfer (DEPT)

