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[Determination of quercetin in the Herba Euphorbiae Humifusae]
Jun-bo Xing1, Cai-hong Shui, Yun Liu
1Institute for Drug and Instrument Control Health Dept GLD of PLA, Beijing 100071, China. junboxing@sohu.com
Objective:
To develop an HPLC method for determining quercetin in Herba Euphorbiae Humifusae.
Method:
ODS column was used with acetonitrile: 0.4% phosphoric acid (40:60) as mobile phase. The detection wavelength was at 370 nm and the flow rate was 1.0 mL.min-1.
Result:
The linearity was obtained over the range of 0.0364-0.1274 microgram (r = 0.9999). The average recovery was 100.71% with relative standard deviation of 2.45%.
Conclusion:
This method can be used for quality control of Herba Euphorbiae Humifusae. It is simple, rapid and reliable.