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Transposition in Lactobacillus delbrueckii subsp. bulgaricus: identification of two thermosensitive replicons and two
Pascale Serror1, Golnar Ilami2, Hichem Chouayekh2
1Unité Recherche Laitière et Génétique Appliquée, INRA, Domaine de Vilvert, 78352 Jouy-en-Josas Cedex, France.
Microbiology (Reading, England)
|June 5, 2003
Abstract:
In this report, it is shown that the rolling circle replicon pG(+)host and the theta replicon pIP501 are thermosensitive in Lactobacillus delbrueckii subsp. bulgaricus (Lactobacillus bulgaricus). Using a pIP501 derivative as a delivery vector for six insertion sequences originating from lactic acid bacteria, it is shown that IS1223 and IS1201 transpose in L. bulgaricus.