Automated pneumococcal MLST using liquid-handling robotics and a capillary DNA sequencer
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Updated: Sep 25, 2026

Automated Robotic Liquid Handling Assembly of Modular DNA Devices
Published on: December 1, 2017
Johanna Jefferies1, Stuart C Clarke, Mathew A Diggle
1Scottish Meningococcus and Pneumococcus Reference Laboratory, North Glasgow University Hospital NHS Trust, Department of Microbiology, Stobhill Hospital, Balornock Rd., Glasgow, G21 3UW, UK.
Abstract:
Multilocus sequence typing (MLST) is used by the Scottish Meningococcus and Pneumococcus Reference Laboratory (SMPRL) as a routine method for the characterization of certain bacterial pathogens. The SMPRL recently started performing MLST on strains of Streptococcus pneumoniae, and here we describe a fully automated method for MLST using a 96-well-format liquid-handling robot and a 96-capillary automated DNA sequencer.
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