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Protein Crystallization for X-ray Crystallography
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Protein Crystallization for X-ray Crystallography

Published on: January 16, 2011

Crystallization and preliminary X-ray characterization of a novel calcium-binding protein AtCBL2 from Arabidopsis

Masamichi Nagae1, Akira Nozawa, Nozomu Koizumi

  • 1Graduate School of Integrated Science, Yokohama City University, 1-7-29 Suehiro-cho, Tsurumi-ku, Yokohama, Kanagawa 230-0045, Japan.

Acta Crystallographica. Section D, Biological Crystallography
|June 5, 2003
PubMed

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X-ray diffraction or XRD is an analytical tool that utilizes X-rays to study ordered structures such as crystalline organic and inorganic samples, polycrystalline materials, proteins, carbohydrates, and drugs.
According to Bragg's law, when X-rays strike the sample positioned on a stage, the rays are  scattered by the electron clouds around the sample atoms. The  X-ray diffraction or scattering is caused by constructive interference of the X-ray waves that reflect off the internal crystal...