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[Antioxidant effect of Ganoderma polysaccharide peptide]
1Department of Pharmacology, School of Basic Medical Sciences, Health Science Center, Peking University, Beijing 100083, China.
Yao Xue Xue Bao = Acta Pharmaceutica Sinica
|June 5, 2003
Summary
Ganoderma polysaccharide peptide (GLPP) demonstrates significant antioxidant effects by reducing oxidative stress and enhancing protective enzyme activity. This peptide offers potential therapeutic benefits against oxidative damage.
Area of Science:
- Biochemistry
- Pharmacology
- Oxidative Stress Research
Background:
- Oxidative modification of low-density lipoprotein (LDL) is a critical factor in cardiovascular disease.
- Reactive oxygen species (ROS) play a significant role in cellular damage and various pathologies.
- Ganoderma lucidum derivatives are explored for their potential health benefits.
Purpose of the Study:
- To investigate the antioxidant properties of Ganoderma polysaccharide peptide (GLPP).
- To elucidate the underlying mechanisms of GLPP's antioxidant action.
- To evaluate GLPP's efficacy in preventing LDL oxidation and ROS-induced injury.
Main Methods:
- In vitro: LDL oxidative modification induced by copper.
- In vivo: ROS-induced injury in mice using alloxan.
- Biochemical assays to measure malondialdehyde (MDA), glutathione peroxidase (GSHpx), superoxide dismutase (SOD), and catalase (CAT) levels.
Main Results:
- GLPP inhibited LDL oxidation and reduced the relative electrophoretic mobility (REM) of oxidized LDL.
- GLPP administration decreased serum and heart MDA concentrations in mice.
- GLPP increased GSHpx activity and decreased SOD levels, while CAT levels remained unchanged.
Conclusions:
- GLPP exhibits notable antioxidant effects both in vitro and in vivo.
- The mechanism involves scavenging ROS and enhancing endogenous antioxidant enzyme activity, particularly GSHpx.
- GLPP shows promise as a therapeutic agent against oxidative stress-related conditions.