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Enzyme-Linked Immunosorbent Assay

Enzyme-Linked Immunosorbent Assay

In 1971, Peter Perlman and Eva Engvall developed an Enzyme-linked immunosorbent assay (ELISA or EIA). ELISA differs from western blot in that the assays are conducted in microtiter plates or in vivo rather than on an absorbent membrane.
There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen. Enzyme-substrate reaction allows the antigen to be visualized or quantified.

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Related Experiment Video

Updated: Jun 26, 2026

Rapid Homogeneous Detection of Biological Assays Using Magnetic Modulation Biosensing System

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Published on: June 14, 2010

Development and application of a pig IL-8 ELISA detection system.

I Splichal¹, Y Muneta, Y Mori

¹Department of Veterinary Microbiology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Yayoi, Tokyo, Japan. splichal@biomed.cas.cz

Journal of Immunoassay & Immunochemistry

|June 5, 2003

Summary

A new pig Interleukin 8 (IL-8) enzyme-linked immunosorbent assay (ELISA) was developed. This assay accurately measures IL-8 in biological samples, offering a faster alternative to traditional methods.

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Area of Science:

Immunology
Biochemistry

Background:

Interleukin 8 (IL-8) is a key chemokine involved in inflammatory processes, particularly neutrophil activation.
Accurate measurement of IL-8 is crucial for understanding and managing inflammatory conditions.

Purpose of the Study:

To develop and validate a specific enzyme-linked immunosorbent assay (ELISA) for quantifying pig Interleukin 8 (IL-8).
To provide a reliable and efficient method for measuring IL-8 concentrations in various biological samples.

Main Methods:

Development of a streptavidin-biotin amplified sandwich ELISA using specific mouse monoclonal antibodies (mAbs) against recombinant pig IL-8.
Validation of the assay for specificity, reproducibility, and working range using recombinant and natural pig IL-8.

Main Results:

The developed pig IL-8 ELISA demonstrated high specificity and reproducibility for both recombinant and natural pig IL-8.
The assay has a working range of 16-1000 pg/mL and requires only 3.5 hours of incubation.
The assay accurately measures IL-8 concentrations in cell culture supernatants and biological fluids.

Conclusions:

The newly developed pig IL-8 ELISA is a sensitive, specific, and rapid method for IL-8 quantification.
This ELISA serves as a valuable alternative to time-consuming and labor-intensive IL-8 bioassays in research and diagnostics.
The assay facilitates the study of inflammatory processes in pigs through precise IL-8 measurement.