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Development and application of a pig IL-8 ELISA detection system

I Splichal1, Y Muneta, Y Mori

  • 1Department of Veterinary Microbiology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Yayoi, Tokyo, Japan. splichal@biomed.cas.cz

Journal of Immunoassay & Immunochemistry
|June 5, 2003
PubMed

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Enzyme-Linked Immunosorbent Assay01:33

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In 1971, Peter Perlman and Eva Engvall developed an Enzyme-linked immunosorbent assay (ELISA or EIA). ELISA differs from western blot in that the assays are conducted in microtiter plates or in vivo rather than on an absorbent membrane.
There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen.  Enzyme-substrate reaction allows the antigen to be visualized or quantified.