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Virulence-associated genes in Escherichia coli isolates from poultry with colibacillosis
Elaine R Delicato1, Benito Guimarães de Brito, Luis Carlos J Gaziri
1Departamento de Microbiologia, Universidade Estadual de Londrina, Campus Universitário, Caixa Postal 6001, 86051-970 Londrina, Paraná, Brazil.
Abstract:
Avian pathogenic Escherichia coli, the causative agent of colibacillosis, harbors several putative virulence genes. In this study we examined by polymerase chain reaction (PCR) the presence of 16 of those genes in 200 colibacillosis isolates from our region. The seven virulence genes iutA, iss, cvaC, tsh, papC, papG and felA were detected significantly more often amongst colibacillosis isolates than in fecal isolates from healthy birds, thereby confirming their worldwide occurrence and possible pathogenic role in colibacillosis. However, several of those genes were not detected in many colibacillosis isolates, and none of them were detected in 27.5% of those isolates, which suggests that variants of those genes and yet undetected virulence factors should be searched for.