Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Electrostatic charge activates inflammatory vanilloid (VR1) receptors.

Bellina Veronesi1, Guangwei Wei, Jin Qi Zeng

  • 1Neurotoxicology Division, Office of Research and Development, National Health and Environmental Effects Research Laboratory, US Environmental Protection Agency, Research Triangle Park, NC, USA.

Neurotoxicology
|June 5, 2003
PubMed
Summary

Negative electrostatic charge on particles can trigger inflammation by activating proton-sensitive receptors. This study shows that negatively charged particles stimulate inflammatory responses in human cells via vanilloid receptors (VR1) and acid-sensitive ion channels (ASICs).

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Physiological and molecular insights into nitrogen rate and planting density interactive regulation of black sesame nitrogen use efficiency, growth, yield, and seed quality.

BMC plant biology·2026
Same author

HNRNPC lactylation promotes pancreatic cancer progression through mediating the alternative splicing of PAK6.

Cancer letters·2025
Same author

Acylation modification-mediated metabolism reprogramming in cancer.

iScience·2025
Same author

Impact of bimekizumab and certolizumab pegol on efficacy, safety and osteoblastic activity in radiographic axial spondyloarthritis: results from a phase IIa, multicentre, randomised, double-blind, exploratory study with PET-CT imaging.

Therapeutic advances in musculoskeletal disease·2024
Same author

Regulation of HNRNP family by post-translational modifications in cancer.

Cell death discovery·2024
Same author

Integrated Lactylome Characterization Reveals the Molecular Dynamics of Protein Regulation in Gastrointestinal Cancers.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2024

Area of Science:

  • Biophysics
  • Cell Biology
  • Toxicology

Background:

  • Neurogenic inflammation involves vanilloid receptors (VR1) and acid-sensitive ion channels (ASICs).
  • Particulate matter (PM) can initiate inflammatory responses.
  • The role of particle charge in activating these pathways is not fully understood.

Purpose of the Study:

  • To investigate if negatively charged synthetic polystyrene micelles (SPM) can activate VR1 and ASICs.
  • To determine if electrostatic charge on SPM induces biological activation in human bronchial-epithelial cells (BEAS-2B).

Main Methods:

  • Synthesized SPM with varying charges (diamino: positive, carboxyl: negative) and measured zeta potential.
  • Exposed BEAS-2B cells to different SPM types.
  • Measured cell responses including membrane depolarization, intracellular calcium ([Ca(2+)](i)) levels, and IL-6 release.

Related Experiment Videos

Main Results:

  • Negatively charged carboxyl-SPM induced membrane depolarization and increased [Ca(2+)](i) and IL-6 release in BEAS-2B cells.
  • These inflammatory effects were reduced by VR1 and ASIC antagonists (capsazepine and amiloride).
  • Carboxyl-SPM, but not diamino-SPM, increased VR1 receptor mRNA expression.

Conclusions:

  • The electrostatic charge on inert particles can stimulate inflammatory changes in human epithelial cells.
  • These inflammatory responses are mediated by acid-sensitive VR1 receptors and ASICs.
  • This highlights a novel mechanism for particle-induced inflammation.