[Study on regulatory effects of interleukin-6 on placental growth factor expression in trophoblast cells]
Wen Peng1, Sen Jiang, Min-sheng Dai
1Department of Obstetrics and Gynecology, Shandong Provincial Hospital, Jinan 250021, China.
Objective:
To investigate the expression pattern of Placental growth factor (PlGF) and regulatory effects of interleukin-6 (IL-6) on PlGF expression in the primary cultured trophoblast cells.
Methods:
Cytotrophoblast cells were collected by trypsin-collagenase digestion and Percoll gradient centrifugation for primary culture. Reverse transcriptase polymerase chain reaction (RT-PCR) were resorted to demonstrate the expression pattern of PlGF mRNA in trophoblast cells cultured in vitro. The effects of IL-6 with different concentration (100, 10, 1 and 0.1 microg/L) and duration (6, 12, 24 and 48 h) on PlGF expression were observed.
Results:
The 185 bp and 248 bp bands of PlGF were showed by RT-PCR. PlGF expression correlated with IL-6. PlGF began to increase at 6 h, and reached the climax at 12 h when recultured with 100 microg/L IL-6.
Conclusions:
PlGF expression has time and dose dependence on IL-6. It may play an important role in early pregnancy.
