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A new mutation of 5-alpha-reductase type 2 (A62E) in a large Egyptian kindred
Mona Hafez1, Inas Mazen, Isis Ghali
1The National Research Center, Cairo University, Cairo, Egypt.
Objective:
To describe the clinical, biological and molecular data in a large Egyptian kindred with 5alpha-reductase deficiency.
Patients And Methods:
Three patients with ambiguous genitalia were referred at the ages of 20, 9 and 2 years, respectively. In all cases, parents were first cousins. Basal and post-HCG stimulation plasma levels of testosterone and dihydrotestosterone were determined. Direct sequencing and restriction site analysis were applied for patient and family study.
Results:
A homozygous alanine to glutamic acid substitution at position 62 (A62E) was found in the three patients. The parents and two XX sisters were heterozygous while a third XX sibling was normal.
Conclusion:
We report a new mutation of the 5alpha-reductase type 2 gene. The presence of this mutation in all studied patients and their parents suggests its causative role in 5alpha-reductase deficiency. Identification of the mutation enabled genetic counselling for three XX individuals.