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Complex Numbers01:29

Complex Numbers

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The real number system cannot represent the square root of a negative number, which restricts solutions for certain equations, such as quadratics with negative discriminants. To address this, the complex number system was developed, introducing the imaginary unit i, where i = √(-1). This extension allows for the representation of all roots, including those involving negative radicands.A complex number is written in the form x + yi, where x and y are real numbers. Here, x represents the...
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Cellular Respiration01:18

Cellular Respiration

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Cellular respiration is a crucial metabolic process through which cells obtain energy from organic substances, mainly glucose, to produce adenosine triphosphate (ATP). This process includes the oxidation of substrates and the transfer of electrons to a separate electron acceptor, facilitating ATP synthesis through a sequence of biochemical reactions.Glycolysis: The Initial StepGlycolysis is the first stage of cellular respiration, occurring in the cytoplasm of both prokaryotic and eukaryotic...
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Protein Complex Assembly02:41

Protein Complex Assembly

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Proteins can form homomeric complexes with another unit of the same protein or heteromeric complexes with different types.  Most protein complexes self-assemble spontaneously via ordered pathways, while some proteins need assembly factors that guide their proper assembly. Despite the crowded intracellular environment, proteins usually interact with their correct partners and form functional complexes.
Many viruses self-assemble into a fully functional unit using the infected host cell to...
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Protein Complexes with Interchangeable Parts01:57

Protein Complexes with Interchangeable Parts

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Groups of proteins may form a complex where each protein in this complex has a different role in the overall execution of the complex’s function. Often some of the proteins in the complex can be replaced by a closely related variant to give a complex that contains many of the same components yet is functionally distinct.
The SCF ubiquitin ligase is a protein complex of five individual proteins. This complex attaches ubiquitin to other target proteins to mark them for degradation. In order...
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Formation of Complex Ions03:45

Formation of Complex Ions

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A type of Lewis acid-base chemistry involves the formation of a complex ion (or a coordination complex) comprising a central atom, typically a transition metal cation, surrounded by ions or molecules called ligands. These ligands can be neutral molecules like H2O or NH3, or ions such as CN− or OH−. Often, the ligands act as Lewis bases, donating a pair of electrons to the central atom. These types of Lewis acid-base reactions are examples of a broad subdiscipline called coordination...
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Cellular Differentiation00:57

Cellular Differentiation

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How does a complex organism such as a human develop from a single cell? It all starts from a single fertilized egg which gives rise to a vast array of cell types, such as nerve cells, muscle cells, and epithelial cells that characterize the adult? Throughout development and adulthood, cellular differentiation leads cells to assume their final morphology and physiology. Differentiation is the process by which unspecialized cells become specialized to carry out distinct functions.
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Related Experiment Video

Updated: Jan 29, 2026

Laser Microirradiation to Study In Vivo Cellular Responses to Simple and Complex DNA Damage
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Seprase complexes in cellular invasiveness.

Wen-Tien Chen1, Thomas Kelly

  • 1Department of Medicine, Division of Neoplastic Diseases, State University of New York at Stony Brook, Stony Brook, NY 11794-8154, USA. wenchen@notes.cc.sunysb.edu

Cancer Metastasis Reviews
|June 6, 2003
PubMed
Summary
This summary is machine-generated.

Type II transmembrane proteases like dipeptidyl peptidase IV (DPP4) and seprase (FAPalpha) function on cell surfaces. Their complexes at invadopodia aid tumor invasion by processing factors and degrading extracellular matrix.

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Area of Science:

  • Biochemistry
  • Cell Biology
  • Oncology

Background:

  • Type II integral serine proteases, including dipeptidyl peptidase IV (DPP4/CD26) and seprase/fibroblast activation protein alpha (FAPalpha), are cell surface-bound enzymes.
  • These proteases, along with related type II transmembrane prolyl serine peptidases, play roles in cellular functions.
  • DPP4 and seprase can form complexes with each other and interact with other membrane molecules, leading to diverse functions.

Purpose of the Study:

  • To investigate the functional significance of cell surface protease complexes, particularly DPP4 and seprase.
  • To understand the role of these protease complexes localized at invadopodia in cellular processes.
  • To elucidate the involvement of these enzymes in tumor-related phenomena such as invasion and metastasis.

Main Methods:

  • The study focuses on the localization and function of type II integral serine proteases.
  • Analysis of protease complex formation and interactions with other membrane-associated molecules.
  • Investigating the enzymatic activity and substrate processing at cell surface protrusions (invadopodia).

Main Results:

  • DPP4 and seprase form complexes that exhibit multiple functions.
  • Localization of these protease complexes at invadopodia was observed.
  • These complexes are involved in processing soluble factors and degrading extracellular matrix components.

Conclusions:

  • Cell surface protease complexes, particularly DPP4 and seprase at invadopodia, are crucial for processing soluble factors and degrading the extracellular matrix.
  • These activities are essential for cell migration and matrix invasion.
  • The findings highlight the role of these proteases in tumor invasion, angiogenesis, and metastasis.