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Related Experiment Videos

Endogenous nitric oxide does not modulate mesenteric mast cell degranulation in rats.

Fábio H Kwasniewski1, Wothan Tavares de Lima, Y S Bakhle

  • 1Department of Immunology, Institute of Biomedical Sciences, University of São Paulo, Brazil.

Biochemical Pharmacology
|June 6, 2003
PubMed
Summary

Endogenous nitric oxide does not cause decreased mast cell degranulation in genetically hypertensive rats (SHR). Inhibiting nitric oxide synthesis with L-NAME did not alter mast cell degranulation in SHR or normotensive rats.

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Area of Science:

  • Physiology
  • Immunology
  • Pharmacology

Background:

  • Genetically hypertensive rats (SHR) exhibit decreased mesenteric mast cell degranulation post-anaphylaxis.
  • Endogenous nitric oxide (NO) was hypothesized to mediate this inhibitory effect.

Purpose of the Study:

  • To investigate the role of endogenous nitric oxide in modulating mast cell degranulation in SHR and normotensive (NT) rats.
  • To determine if NO inhibition affects anaphylaxis-induced mast cell degranulation or passive cutaneous anaphylaxis (PCA).

Main Methods:

  • Rats (SHR and NT) were sensitized to ovalbumin and challenged.
  • Mast cell degranulation assessed via microvascular permeability (Evans blue dye extravasation) in duodenum, mesentery, and skin.
  • Mesenteric mast cell degranulation also evaluated using light microscopy (toluidine blue staining).

Related Experiment Videos

  • Nitric oxide synthesis inhibition using L-NAME (Nω-nitro-L-arginine methyl ester).

    • Main Results:

    • L-NAME pretreatment did not alter dye extravasation after immunological challenge or compound 48/80 in the mesentery of SHR or NT rats.
    • Passive cutaneous anaphylaxis (PCA) was defective in SHR but unaffected by L-NAME.
    • L-NAME did not modulate normal PCA reactions in NT rats.

    Conclusions:

    • Endogenous nitric oxide does not cause the defective mast cell degranulation observed in SHR.
    • Nitric oxide inhibition does not modulate mesenteric or skin mast cell degranulation in normotensive rats during anaphylaxis.