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A simple and rapid fluorescence-based immunoassay for the detection of staphylococcal enterotoxin B
Akbar S Khan1, Cheng J Cao, Roy G Thompson
1US Army Edgewood Chemical Biological Centre, AMSSB-RRT, E3330/143, 5183 Blackhawk Road, APG, MD 21010-5424, USA. akbar.khan@sbccom.apgea.army.mil
Abstract:
The bioterrorism threat is perceived to be a real challenge to our nation's security. This threat has necessitated the design of better and faster assays for the detection of biothreat agents including staphylococcal enterotoxin B (SEB), a causative agent of food poisoning. This study describes a simple, fast and highly sensitive fluorescence-based immunoassay, in which the antibody is fluorescently-labeled for use in this assay. Use of labeled antibodies resulted in very low level of detection of SEB, 100 pg/well. This method is four times faster than classical and conventional enzyme-linked immunosorbent assay (ELISA).
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