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Matrix metalloproteinase expression in breast cancer
John E Bartsch1, Edgar D Staren, Hubert E Appert
1Department of Surgery, Medical College of Ohio, 3065 Arlington Avenue, Toledo, OH 43614, USA.
The Journal of Surgical Research
|June 6, 2003
Summary
Breast tumor cells express matrix metalloproteinases (MMPs) constitutively. Agents like TPA and Con-A up-regulate MMPs, with mitogen-activated protein kinase (MAPK) signaling playing a key role in this regulation.
Area of Science:
- Oncology
- Molecular Biology
- Biochemistry
Background:
- Matrix metalloproteinases (MMPs) are implicated in cancer invasion and metastasis.
- Understanding constitutive and inducible MMP expression in breast tumors is crucial.
Purpose of the Study:
- To investigate constitutive MMP expression in breast tumor cell lines.
- To identify agents that up-regulate MMP expression.
- To explore the role of signaling pathways in MMP regulation.
Main Methods:
- Evaluated MMPs 1-16 expression in MDA-MB-231, T47D, and MCF-7 cell lines using RT-PCR and zymography.
- Tested effects of TPA, Con-A, RGD peptide, ECM components, and anti-integrin antibodies on MMP expression.
- Utilized mitogen-activated protein kinase inhibitors (MAPK-I) to assess signal transduction pathways.
Main Results:
- Constitutive expression of MMPs 1, 2, 7-11, 13, 14, and 16 observed in tumor cell lines.
- TPA, Con-A, and RGD peptide increased expression of MMPs 1, 2, 9, and 10.
- MAPK inhibitors affected TPA-induced MMP-9 up-regulation but not constitutive expression.
Conclusions:
- Breast tumor cell lines exhibit constitutive expression of various MMPs.
- MMP expression is inducible by agents like Con-A, RGD, and TPA.
- MAPK signaling pathways are involved in the regulation of inducible MMP expression.