Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Benzene transformation in nitrifying batch cultures.

A Zepeda1, A-C Texier, J Gomez

  • 1Departamento de Biotecnología, Div. CBS, Universidad Autónoma Metropolitana-Iztapalapa, Av. San Rafael Atlixco 186, C.P. 09340, México, DF.

Biotechnology Progress
|June 7, 2003
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Nitrification activity in the presence of 2-chlorophenol using whole nitrifying cells and cell-free extracts: batch and SBR assays.

3 Biotech·2023
Same author

Measurement of the Fluctuations in the Number of Muons in Extensive Air Showers with the Pierre Auger Observatory.

Physical review letters·2021
Same author

Features of the Energy Spectrum of Cosmic Rays above 2.5×10^{18}  eV Using the Pierre Auger Observatory.

Physical review letters·2020
Same author

Constraints on Lorentz Invariance Violation from HAWC Observations of Gamma Rays above 100 TeV.

Physical review letters·2020
Same author

Publisher Correction: Very-high-energy particle acceleration powered by the jets of the microquasar SS 433.

Nature·2018
Same author

Very-high-energy particle acceleration powered by the jets of the microquasar SS 433.

Nature·2018

Benzene significantly inhibits nitrifying activity in wastewater sludge, with higher concentrations leading to reduced nitrate formation. However, nitrifying bacteria can degrade benzene, suggesting potential for combined nitrogen and benzene removal.

Area of Science:

  • Environmental microbiology
  • Bioremediation
  • Wastewater treatment

Background:

  • Nitrifying bacteria are crucial for nitrogen removal in wastewater treatment.
  • Benzene is a common industrial pollutant that can be toxic to microorganisms.
  • Understanding the interaction between benzene and nitrifying sludge is vital for effective bioremediation.

Purpose of the Study:

  • To evaluate the impact of benzene on the nitrifying activity of sludge.
  • To investigate the degradation pathways of benzene by nitrifying consortia.
  • To explore the potential for combined nitrogen and benzene removal from wastewater.

Main Methods:

  • Batch culture experiments were conducted to assess nitrifying activity.
  • Benzene concentrations were varied to determine inhibition levels and degradation rates.

Related Experiment Videos

  • Metabolic products of benzene degradation were analyzed to elucidate pathways.

    • Main Results:

    • Benzene at 10 mg/L inhibited nitrate formation by 53%, while 5 mg/L showed no significant inhibition.
    • Specific rates of nitrate production decreased with increasing benzene concentration.
    • Benzene was degraded to phenol and subsequently to acetate at concentrations up to 10 mg/L.
    • At higher concentrations (20-30 mg/L), benzene was converted to other intermediates.

    Conclusions:

    • Nitrifying sludge activity is sensitive to benzene contamination.
    • Nitrifying consortia can degrade benzene, with specific pathways observed.
    • The combined nitrifying and denitrifying system shows promise for simultaneous removal of nitrogen and benzene from contaminated wastewater.