Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Multilocus sequence typing system for group B streptococcus.

Nicola Jones1, John F Bohnsack, Shinji Takahashi

  • 1Nuffield Department of Clinical Laboratory Sciences, Institute for Molecular Medicine, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom. nicola.jones@ndcls.ox.ac.uk

Journal of Clinical Microbiology
|June 7, 2003
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Selective IgA Deficiency and COVID-19 Outcomes: A Nationwide Retrospective Cohort Study.

Journal of clinical medicine·2026
Same author

How should immune checkpoint inhibitor myocarditis be treated?

Cardio-oncology (London, England)·2025
Same author

Avirulent <i>Pseudomonas aeruginosa</i> T3SS-negative strains belonging to Clade 5 produce variable quantities of secondary metabolites.

microLife·2025
Same author

Splenic artery embolization for the management of severe life-threatening warm autoimmune hemolytic anemia.

Hematology (Amsterdam, Netherlands)·2025
Same author

Splenic Embolization in Refractory Immune Thrombocytopenia.

European journal of haematology·2025
Same author

Implementation study of the CARRA Uveitis Consensus Treatment Plans: feasibility for clinical practice and applicability for research.

Pediatric rheumatology online journal·2024
Same journal

Ebola laboratory preparedness at frontline hospitals: can we or can't we?

Journal of clinical microbiology·2026
Same journal

Reporting macrolide-resistant <i>Mycoplasma pneumoniae</i>: a diagnostic obligation?

Journal of clinical microbiology·2026
Same journal

Diagnostic value of HHV-6A/B genotyping in immunocompromised adults.

Journal of clinical microbiology·2026
Same journal

Multicenter performance evaluation of the Simplexa <i>C. auris</i> Direct assay for the detection of <i>Candida auris</i> colonization in bilateral axilla/groin swabs.

Journal of clinical microbiology·2026
Same journal

Comparison of blood culture contamination rates with standard practice versus two blood diversion devices at a single institution.

Journal of clinical microbiology·2026
Same journal

Risk assessment and mitigation of hepatitis C virus RNA carryover contamination in a reflex testing algorithm.

Journal of clinical microbiology·2026
See all related articles

A new multilocus sequence typing (MLST) system for group B streptococcus (GBS) identified 29 sequence types (STs). Specific STs were linked to asymptomatic carriage and neonatal invasive infections, aiding GBS population biology studies.

Area of Science:

  • Microbiology
  • Genetics
  • Epidemiology

Background:

  • Group B Streptococcus (GBS) is a significant human pathogen.
  • Understanding GBS population structure is crucial for disease control.

Purpose of the Study:

  • To develop and validate a multilocus sequence typing (MLST) system for GBS.
  • To characterize the genetic diversity of GBS strains using MLST.
  • To investigate the association between GBS sequence types and clinical outcomes.

Main Methods:

  • Developed an MLST system targeting seven housekeeping genes in GBS.
  • Amplified and sequenced gene fragments from 152 diverse human GBS isolates.
  • Analyzed allelic profiles to determine sequence types (STs).
  • Correlated STs with capsular serotypes and clinical data (carriage vs. invasive disease).

Related Experiment Videos

Main Results:

  • Identified 29 distinct GBS sequence types (STs) among 152 isolates.
  • 66% of isolates clustered into four major STs.
  • ST-1 and ST-19 were associated with asymptomatic carriage.
  • ST-17 represented a homogeneous clone of serotype III GBS strongly linked to neonatal invasive infections.
  • Evidence of recombination at the capsular locus was suggested by shared STs among different serotypes.

Conclusions:

  • The developed GBS MLST system is a valuable tool for characterizing GBS population diversity.
  • Specific GBS STs are associated with distinct epidemiological profiles and clinical outcomes.
  • MLST provides insights into GBS evolution, including potential capsular recombination.