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Multiplex PCR assay for identification of human diarrheagenic Escherichia coli
Claudia Toma1, Yan Lu, Naomi Higa
1Department of Bacteriology, Faculty of Medicine, University of the Ryukyus, Nishihara, Okinawa 903-0215, Japan. k950417@med.u-ryukyu.ac.jp
Journal of Clinical Microbiology
|June 7, 2003
Abstract:
A multiplex PCR assay for the identification of human diarrheagenic Escherichia coli was developed. The targets selected for each category were eae for enteropathogenic E. coli, stx for Shiga toxin-producing E. coli, elt and est for enterotoxigenic E. coli, ipaH for enteroinvasive E. coli, and aggR for enteroaggregative E. coli. This assay allowed the categorization of a diarrheagenic E. coli strain in a single reaction tube.