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Updated: Sep 25, 2026

Analysis of the Lipid Composition of Mycobacteria by Thin Layer Chromatography
Published on: April 16, 2021
Characterization of the Manila family of Mycobacterium tuberculosis
James T Douglas1, Lishi Qian, Jaime C Montoya
1Department of Microbiology, University of Hawaii, Honolulu, Hawaii 96822, USA. jdouglas@hawaii.edu
Abstract:
Forty-eight Mycobacterium tuberculosis strains were obtained from patients living in metropolitan Manila, Republic of the Philippines. Three molecular typing methods, IS6110 restriction fragment length polymorphism, spoligotyping, and DNA sequencing of the oxyR, gyrA, and katG loci, established that these strains have restricted diversity and are members of a related genetic group of organisms. Comparison of the DNA fingerprint patterns with those in international databases confirmed the uniqueness of this group of isolates, which we designate the Manila family of M. tuberculosis.
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