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Related Experiment Videos

Corneal endothelial cell damage from glaucoma drainage device materials.

K Sheng Lim1

  • 1Institute of Opthalmology, University of Brighton, Brighton, United Kingdom. shenglim@email.com

Cornea
|June 7, 2003
PubMed
Summary

Phosphorylcholine polymer-coated polymethylmethacrylate (PC-PMMA) significantly reduced corneal endothelial cell damage compared to silicone and standard PMMA in glaucoma drainage device materials. This suggests PC coating may prevent endothelial failure after GDD implantation.

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Area of Science:

  • Ophthalmology
  • Biomaterials Science
  • Cell Biology

Background:

  • Glaucoma drainage devices (GDDs) are used to manage intraocular pressure.
  • Endothelial cell damage is a potential complication following GDD implantation.
  • Investigating biomaterials for GDDs to minimize ocular tissue damage is crucial.

Purpose of the Study:

  • To compare the endothelial cell damage caused by silicone, polymethylmethacrylate (PMMA), and phosphorylcholine polymer-coated PMMA (PC-PMMA) used in GDDs.
  • To evaluate the efficacy of PC coating in reducing material-induced endothelial trauma.

Main Methods:

  • Standardized discs of silicone, PMMA, and PC-PMMA were applied to cultured bovine corneal endothelial (BCE) cell monolayers.
  • A standardized weight was applied to each disc for 5 minutes.

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  • Trypan blue staining quantified the area of endothelial cell damage, calculated as a damage coefficient.

    • Main Results:

    • PMMA caused significant endothelial cell damage (5.95% +/- 4.60%).
    • Silicone showed less damage (3.08% +/- 2.68%).
    • PC-PMMA exhibited minimal damage (0.15% +/- 0.25%), significantly lower than PMMA (p=0.04).

    Conclusions:

    • PC coating technology significantly reduces corneal endothelial cell damage.
    • PC-PMMA demonstrates a favorable safety profile for GDD materials.
    • PC coating may be effective in preventing corneal endothelial failure after GDD implantation.