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Updated: Sep 25, 2026
Tumor Transplantation for Assessing the Dynamics of Tumor-Infiltrating CD8+ T Cells in Mice
Published on: June 12, 2021
Effects of the CDT6/ANGX gene on tumour growth in immune competent mice
Diane Bouïs1, Geke A P Hospers, Coby Meijer
1Department of Medical Oncology, University Hospital Groningen, P.O. Box 30001, 9700 RB Groningen, The Netherlands.
Background:
Cornea-derived transcript 6 (CDT6, also known as AngX) has been described to inhibit tumour growth in a human melanoma growing in nude mice. In another report, however, enhancement of tumour growth in comparable circumstances occurred. We therefore studied the effect of the same gene in different circumstances, using an immune competent mouse model.
Materials And Methods:
In this report we describe the generation of a stably CDT6-expressing clone of the murine melanoma cell line B16-F10. These cells were implanted in female C57/B16 mice with empty vector transfected cells as control.
Results:
We found no significant inhibition or stimulation of either lag-time or doubling-time of the tumours. In addition no effect of the CDT6 gene product was found on proliferation of endothelial cells in vitro.
Conclusion:
In contrast to an immune deficient mouse model, no anti-tumour effect could be detected with the CDT6 gene product in an immune competent mouse model.
Insights
Cornea-derived transcript 6 (CDT6) did not inhibit melanoma growth in immune-competent mice. This study found no anti-tumor effect of CDT6 in a C57/B16 mouse model, contrasting previous findings.
Area of Science:
- Oncology
- Molecular Biology
- Immunology
Background:
- Cornea-derived transcript 6 (CDT6/AngX) has conflicting reports regarding its effect on tumor growth.
- Previous studies showed CDT6 inhibiting or enhancing melanoma growth in immune-deficient mice.
Purpose of the Study:
- To investigate the role of CDT6 in tumor growth within an immune-competent mouse model.
- To reconcile conflicting data on CDT6's anti-tumor potential.
Main Methods:
- Generated a stable CDT6-expressing clone of the murine melanoma cell line B16-F10.
- Implanted CDT6-expressing B16-F10 cells into immune-competent C57/B16 mice.
- Utilized empty vector transfected cells as controls and assessed tumor growth parameters.
Main Results:
- No significant inhibition or stimulation of tumor lag-time or doubling-time was observed.
- CDT6 gene product showed no effect on endothelial cell proliferation in vitro.
- The anti-tumor effect of CDT6 was not detected in this immune-competent model.
Conclusions:
- CDT6 does not exhibit an anti-tumor effect in an immune-competent mouse model.
- The tumor microenvironment, specifically the immune system, may influence CDT6's role.
- Further research is needed to understand CDT6's function in different biological contexts.
