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[Study on denitrification using different carbon sources].

Youming Tan1, Qifang Luo

  • 1Institute of Environmental Medicine, Tongji Medical College, Huazhong University of Science & Technology, Wuhan 430030, China.

Wei Sheng Yan Jiu = Journal of Hygiene Research
|June 10, 2003
PubMed
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Immobilized denitrifier effectively reduces nitrate using various carbon sources like glucose and cane sugar. This method achieves over 96% denitrification efficiency in laboratory tests and shows promise for treating nitrate-enriched water.

Area of Science:

  • Environmental Science
  • Microbiology
  • Water Treatment

Background:

  • Nitrate contamination in water bodies poses significant environmental and health risks.
  • Denitrification is a crucial biological process for removing nitrate from wastewater.
  • Immobilized microbial systems offer enhanced stability and efficiency for bioremediation.

Purpose of the Study:

  • To evaluate the efficacy of immobilized denitrifier in reducing nitrate using different exogenous carbon sources.
  • To assess the performance of immobilized denitrifier in treating nitrate-enriched lake water.
  • To investigate the utilization of natural carbon sources by immobilized denitrifier cells.

Main Methods:

  • Bench scale batch tests were performed to assess denitrification rates with glucose, cane sugar, methanol, and acetic acid.

Related Experiment Videos

  • Hydraulic residence time was maintained at 6 hours to determine denitrification efficiency.
  • Real lake water samples were treated to evaluate the immobilized denitrifier's performance with natural carbon sources.

    • Main Results:

    • All tested carbon sources (glucose, cane sugar, methanol, acetic acid) were utilized by immobilized bacteria.
    • Denitrification rates exceeded 96% with a 6-hour hydraulic residence time.
    • Methanol resulted in a lower average denitrifying velocity compared to other carbon sources.
    • Dissimilatory reduction to ammonium was minimal (<5% of reduced nitrate).
    • Over 90% nitrate reduction was achieved in treated lake water, utilizing >20% of natural carbon sources.

    Conclusions:

    • Immobilized denitrifier is a highly effective technology for nitrate removal from water.
    • The choice of carbon source influences denitrification velocity, with methanol being less efficient.
    • The process is robust, with minimal undesirable byproducts like ammonium.
    • This method shows potential for practical application in treating nitrate-polluted water bodies.