Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

[Studies on protein-based identification method of genetically modified capsicum].

Jianjun Liu1, Pingjian Deng, Shisong Fang

  • 1Shenzhen Disease Prevention and Control Center, Shenzhen 518020, China.

Wei Sheng Yan Jiu = Journal of Hygiene Research
|June 10, 2003
PubMed
Summary

A new detection system for genetically modified foods (GMF) uses cecropin BD gene expression in peppers. This method accurately verifies foreign protein expression and safety, ensuring GMF integrity.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Resveratrol promotes regression of renal carcinoma cells via a renin-angiotensin system suppression-dependent mechanism.

Oncology letters·2017
Same author

Impact of systemic inflammation on gastric cancer outcomes.

PloS one·2017
Same author

Genome-Wide Association Study Meta-Analysis of Long-Term Average Blood Pressure in East Asians.

Circulation. Cardiovascular genetics·2017
Same author

Macrophages induce AKT/β-catenin-dependent Lgr5<sup>+</sup> stem cell activation and hair follicle regeneration through TNF.

Nature communications·2017
Same author

A rapid, accurate and sensitive method with the new stable isotopic tags based on microwave-assisted dispersive liquid-liquid microextraction and its application to the determination of hydroxyl UV filters in environmental water samples.

Talanta·2017
Same author

A Low-Frequency Inactivating <i>AKT2</i> Variant Enriched in the Finnish Population Is Associated With Fasting Insulin Levels and Type 2 Diabetes Risk.

Diabetes·2017

Area of Science:

  • Biotechnology
  • Food Safety
  • Molecular Biology

Background:

  • Assessing genetically modified foods (GMF) safety relies on detecting foreign protein expression.
  • The cecropin BD gene from capsicum offers a potential marker for GMF detection.

Purpose of the Study:

  • To establish a protein-based detection system for evaluating the safety and expression of foreign target proteins in GMF.
  • To develop a method for identifying the cecropin D protein expressed in transgenic capsicum.

Main Methods:

  • Utilized hemolymph from immunized Antheraea pernyi pupae as a standard.
  • Employed CM-Sepharose-FF ion-exchange chromatography for protein purification.
  • Identified the purified protein through antibacterial activity assays, electrophoresis, biological auto-photography, and MALDI-TOF mass spectrometry.

Related Experiment Videos

Main Results:

  • The foreign target protein expressed in transgenic capsicum matched the standard protein in physical and chemical properties, antibacterial activity, and molecular weight.
  • Confirmed the real and expected expression of the target gene in capsicum.
  • Established feasible methods for the separation, purification, and identification of cecropin D.

Conclusions:

  • The developed detection method is easy to operate, fast, and feasible for identifying target proteins in GM-capsicum.
  • The study validates the expression and safety of foreign proteins in GMF using a protein-based detection system.