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Related Concept Videos

Tonicity in Plants00:53

Tonicity in Plants

Tonicity describes the capacity of a cell to lose or gain water. It depends on the quantity of solute that does not penetrate the membrane. Tonicity delimits the magnitude and direction of osmosis and results in three possible scenarios that alter the volume of a cell: hypertonicity, hypotonicity, and isotonicity. Due to differences in structure and physiology, tonicity of plant cells is different from that of animal cells in some scenarios.Plants and Hypotonic EnvironmentsUnlike animal cells,...
Tonicity in Plants01:20

Tonicity in Plants

Plant cells maintain appropriate osmotic balance in extreme conditions. For instance, plants in dry environments store water in vacuoles, limit the opening of their stoma, and have thick, waxy cuticles to prevent unnecessary water loss. Some species of plants that live in salty environments store salt in their roots. As a result, water osmosis occurs in the root from the surrounding soil.
Tonicity
Tonicity describes the capacity of a cell to lose or gain water depending on the solute...
Inductively Coupled Plasma–Mass Spectrometry (ICP–MS): Overview01:19

Inductively Coupled Plasma–Mass Spectrometry (ICP–MS): Overview

In inductively coupled plasma–mass spectrometry (ICP–MS), an inductively coupled plasma (ICP) torch is used as an atomizer and ionizer. Solid samples are dissolved and volatilized before being introduced into the high-temperature argon plasma, while solution samples are nebulized and passed through the high-temperature argon plasma. Plasma dissociates the analytes and ionizes their component atoms to form a mixture of positive ions and molecular species. The positive ions are then passed on to...
Gas Chromatography: Types of Detectors-I01:21

Gas Chromatography: Types of Detectors-I

There are different types of detectors used in gas chromatography, each with its own specific properties that make it suitable for detecting certain types of analytes. The most commonly used detectors in GC are thermal conductivity detector (TCD), flame ionization detector (FID), and electron capture detector (ECD).
TCD is the earliest and most widely used detector that operates by measuring the changes in the thermal conductivity of the carrier gas. When a sample compound enters the detector,...
Ion-Exchange Chromatography01:09

Ion-Exchange Chromatography

Ion-exchange chromatography, or IEC, is a technique for separating ions based on their affinity for the stationary phase. The stationary phase is a cross-linked polymer resin with covalently attached ionic functional groups. The functional groups can be either positively charged (cation exchangers) or negatively charged (anion exchangers). A cation exchanger consists of a polymeric anion and active cations, while an anion exchanger is a polymeric cation with active anions. The choice of...
Capillary Electrophoresis: Instrumentation01:20

Capillary Electrophoresis: Instrumentation

Capillary electrophoresis instrumentation typically consists of several key components. A high-voltage power supply generates the electric field necessary for the separation by connecting to an anode (the positively charged electrode) and a cathode (the negatively charged electrode) located in buffer reservoirs at each end of the capillary tube. The system includes a sample vial, a fused silica capillary tube coated with polyimide for mechanical strength through which the sample components...

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Updated: Jun 25, 2026

Using Capillary Electrophoresis to Quantify Organic Acids from Plant Tissue: A Test Case Examining Coffea arabica Seeds
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Using Capillary Electrophoresis to Quantify Organic Acids from Plant Tissue: A Test Case Examining Coffea arabica Seeds

Published on: November 12, 2016

Ionic content in plant extracts determined by ion chromatography with conductivity detection.

Tommaso R I Cataldi1, Giovanna Margiotta, Antonella Del Fiore

  • 1Dipartimento di Chimica, Università degli Studi della Basilicata, Via N. Sauro, 85-85100 Potenza, Italy. cataldi@unibas.it

Phytochemical Analysis : PCA
|June 10, 2003
PubMed
Summary
This summary is machine-generated.

This study details a simple ion chromatography method to measure vegetable ion content. Results show significant differences in ionic profiles, with potassium and nitrate being common ions across samples.

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Extraction and Quantification of Soluble, Radiolabeled Inositol Polyphosphates from Different Plant Species using SAX-HPLC

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Area of Science:

  • Agricultural Science
  • Analytical Chemistry
  • Plant Physiology

Background:

  • Understanding the ionic composition of vegetables is crucial for nutritional and agricultural applications.
  • Variations in ion content can impact plant health, crop yield, and human nutrition.
  • Accurate and efficient methods for ionic analysis in plant tissues are needed.

Purpose of the Study:

  • To establish a straightforward ion chromatography method for determining ionic content in diverse vegetable samples.
  • To compare the ionic profiles of different vegetable tissues, including leaves, cotyledons, and roots.
  • To identify major and minor inorganic and organic ions present in the selected vegetable species.

Main Methods:

  • Ion chromatography with suppressed conductivity detection was employed for ionic analysis.
  • Vegetable samples (cucumber, watermelon, zucchini, olive) were extracted at room temperature.
  • Chromatographic profiles were analyzed to quantify anions (Cl-, NO3-, HPO4(2-), SO4(2-), malate, oxalate) and cations (Na+, NH4+, K+, Mg2+, Ca2+).

Main Results:

  • Significant variations in ionic content were observed across different vegetable tissues and species.
  • Nitrate (NO3-), chloride (Cl-), and potassium (K+) were the predominant ions in most samples.
  • Olive roots showed high oxalate content and a notable presence of sodium (Na+), while K+ was the main cation overall.

Conclusions:

  • The developed ion chromatography method is effective for analyzing ionic content in vegetable samples.
  • Vegetable ionic composition varies considerably, with specific ions like oxalate and Na+ accumulating in certain tissues (e.g., olive roots).
  • This research provides valuable data on the ionic profiles of common vegetables, contributing to plant science and nutritional studies.