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A thrombin-cross-reactive anticardiolipin antibody binds to and inhibits the anticoagulant function of activated

Kwan-Ki Hwang1, Cheng-De Yang, Weihong Yan

  • 1Medicine/Rheumatology, University of California, Los Angeles, CA 90095-1670, USA. hwangkk@ucla.edu

Arthritis and Rheumatism
|June 10, 2003
PubMed

Insights

Some anticardiolipin antibodies (aCL) bind to protein C (PC) and activated PC (APC). One specific aCL inhibited APC

Area of Science:

  • Immunology
  • Hematology
  • Thrombosis Research

Background:

  • Anticardiolipin antibodies (aCL) are associated with thrombosis.
  • The interaction of aCL with protein C (PC) and activated PC (APC) is not fully understood.

Purpose of the Study:

  • To investigate if thrombin-reactive aCL bind to PC and/or APC.
  • To determine if these binding aCL affect PC activation or APC function.

Main Methods:

  • Studied patient-derived monoclonal aCL reactivity with PC and APC.
  • Assessed the impact of reactive antibodies on PC activation and APC activity in plasma.

Main Results:

  • Five of five thrombin-reactive monoclonal aCL bound to PC and APC.
  • One antiprothrombin antibody with aCL activity also bound to PC and APC.
  • One antibody (CL15) inhibited the anticoagulant function of APC.

Conclusions:

  • Thrombin-reactive aCL frequently bind to PC and APC in antiphospholipid syndrome.
  • Some APC-reactive aCL may inhibit APC's anticoagulant function, potentially promoting thrombosis.
Abstract

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