Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Dynamic changes in gene expression during human trophoblast differentiation.

Stuart Handwerger1, Bruce Aronow

  • 1Department of Endocrinology, Children's Hospital Research Foundation, Cincinnati, Ohio 45229-3039, USA. stuart.handwerger@chmcc.org

Recent Progress in Hormone Research
|June 11, 2003
PubMed
Summary

Human placental development involves complex gene regulation. This study reveals "categorical reprogramming," where simultaneous gene induction and repression drive trophoblast differentiation, impacting cell structure, metabolism, and communication.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Editor's Note: Myc-Transformed Epithelial Cells Down-Regulate Clusterin, Which Inhibits Their Growth in Vitro and Carcinogenesis in Vivo.

Cancer research·2026
Same author

Cross-Architecture Knowledge Distillation for Histopathological Image Analysis.

IEEE access : practical innovations, open solutions·2026
Same author

Investigating the oncogenic role of aberrant EZH2 in hepatoblastoma.

Scientific reports·2026
Same author

KAT5 regulates neurodevelopmental states associated with G0-like populations in glioblastoma.

Nature communications·2025
Same author

Multimodal Ensemble Fusion Deep Learning Using Histopathological Images and Clinical Data for Glioma Subtype Classification.

IEEE access : practical innovations, open solutions·2025
Same author

Advancements in automated nuclei segmentation for histopathology using you only look once-driven approaches: A systematic review.

Computers in biology and medicine·2025

Area of Science:

  • Reproductive biology and developmental genetics.
  • Cellular and molecular biology.
  • Genomics and transcriptomics.

Background:

  • Human placental differentiation is a complex process with poorly understood genetic underpinnings.
  • Understanding gene regulation during placental development is crucial for reproductive health.
  • Previous studies lacked comprehensive analysis of dynamic gene expression during trophoblast differentiation.

Purpose of the Study:

  • To identify genes dynamically regulated during human trophoblast differentiation using an in vitro model.
  • To elucidate the patterns and functional categories of gene expression changes during placental development.
  • To investigate the role of "categorical reprogramming" in differentiation.

Main Methods:

  • Utilized DNA microarray analysis (Incyte Human GEM V) to profile gene expression over 9 days.

Related Experiment Videos

  • Employed K-means clustering to identify distinct kinetic patterns of gene induction and repression.
  • Classified dynamically regulated genes into functional categories (e.g., cell structure, metabolism, gene regulation).

    • Main Results:

    • Identified 141 induced and 256 downregulated genes ( >2-fold change) during trophoblast differentiation.
    • Observed distinct kinetic patterns of gene expression changes, tightly coupled to morphological differentiation.
    • Found evidence of "categorical reprogramming" where genes within the same functional category are simultaneously activated and repressed.
    • Observed similar categorical reprogramming in in vitro decidualized human fibroblasts.

    Conclusions:

    • Trophoblast differentiation is characterized by "categorical reprogramming," involving coordinated induction and repression of functionally related genes.
    • This simultaneous regulation enhances the functional capacity of induced genes by diminishing synthesis of others in the same category.
    • Suggests a fundamental role for simultaneous mRNA induction and repression in differentiation processes across different cell types.