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Updated: Sep 25, 2026

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Published on: August 24, 2011
Facile and rapid high-performance liquid chromatography method for simultaneous determination of allopurinol and
1Department of Pharmaceutical Science, Akita University Hospital, 1-1-1 Hondo, Akita 010-8543, Japan.
Objective:
To develop a rapid and sensitive assay for the simultaneous determination of allopurinol and oxypurinol in serum.
Method:
High-performance liquid chromatography (HPLC) with UV-detection. Sample preparation consists of protein precipitation by an addition of trichloracetic acid.
Results:
Percentage recovery and intra-assay coefficient of variation (CV%) for allopurinol were 97.4-101.3 and 0.66-5.13, respectively, in the concentration range 0.5-5.0 microg/mL. For oxypurinol, the percentage recovery and the intra-assay CV% were 93.2-98.1 and 0.88-5.62, respectively, in the concentration ranges 0.4-20 microg/mL. There was no interference of endogenous compounds in this assay.
Conclusion:
This method is useful for routine therapeutic drug monitoring of allopurinol in a clinical setting.
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