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Induction and Assessment of Class Switch Recombination in Purified Murine B Cells
09:49

Induction and Assessment of Class Switch Recombination in Purified Murine B Cells

Published on: August 14, 2010

Visualization of the genesis and fate of isotype-switched B cells during a primary immune response

Kathryn A Pape1, Valerie Kouskoff, David Nemazee

  • 1Department of Microbiology and Center for Immunology, University of Minnesota Medical School, MMC334, 420 Delaware St. S.E., Minneapolis, MN 55455,USA. papex001@umn.edu

The Journal of Experimental Medicine
|June 11, 2003
PubMed

The life history of isotype-switched B cells is unclear, in part, because of an inability to detect rare antigen-specific B cells at early times during the immune response. To address this issue, a small population of B cells carrying targeted antibody transgenes capable of class switching was monitored in immunized mice. After contacting helper T cells, the first switched B cells appeared in follicles rather than in the red pulp, as was expected. Later, some of the switched B cells transiently occupied the red pulp and marginal zone, whereas others persisted in germinal centers (GCs). Antigen-experienced IgM B cells were rarely found in GCs, indicating that these cells switched rapidly after entering GCs or did not persist in this environment.

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