Related Experiment Videos
Ureteral function is modulated by a local renin-angiotensin system.
William F Santis1, Craig A Peters, Subbarao V Yalla
1Division of Urology, Surgical Service, Veterans Affairs Boston Healthcare System, 1400 VFW Parkway, West Roxbury, MA 02132, USA.
The Journal of Urology
|June 11, 2003
Summary
The ureter contains a local renin-angiotensin system that modulates smooth muscle function. Angiotensin II (AngII) influences ureteral contractility, impacting both spontaneous and stimulated contractions.
Area of Science:
- Urology
- Physiology
- Pharmacology
Background:
- Ureteral smooth muscle modulation mechanisms require further elucidation.
- Angiotensin II (AngII) is known to modulate contractility in other smooth muscle types.
Purpose of the Study:
- To determine the presence of a local ureteral renin-angiotensin system.
- To characterize the functional role of AngII in ureteral smooth muscle contractility.
Main Methods:
- RT-PCR to detect mRNA expression of angiotensinogen, renin, ACE, and AT1 receptor.
- Immunohistochemistry to localize AngII in ureteral tissue.
- Tissue bath studies with AngII receptor antagonist (losartan) to assess effects on ureteral contractions.
Main Results:
- Detected mRNA for key renin-angiotensin system components and AT1 receptor in human ureter.
- Demonstrated AngII presence in ureteral smooth muscle and blood vessels.
- Losartan dose-dependently reduced spontaneous and electrically stimulated ureteral contractions.
Conclusions:
- Evidence supports a local renin-angiotensin system within the ureter.
- AngII significantly modulates ureteral smooth muscle contractility.
- This local system may play a crucial role in normal and abnormal ureteral function.