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Related Experiment Videos

Adenovirus endocytosis.

Oliver Meier1, Urs F Greber

  • 1Zoologisches Institut der Universität Zürich, Winterthurerstrasse 190, 8057 Zürich, Switzerland.

The Journal of Gene Medicine
|June 11, 2003
PubMed
Summary

Human adenoviruses (Ad) use clathrin-mediated endocytosis for entry into respiratory cells. Macropinocytosis also plays a role, especially when Ad is targeted to other cell types, aiding infection.

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Area of Science:

  • Cell Biology
  • Virology
  • Molecular Biology

Background:

  • Pathogen entry into host cells utilizes diverse mechanisms including endocytosis.
  • Human adenoviruses (Ad) infect cells via specific receptor interactions and endocytic pathways.

Purpose of the Study:

  • To investigate the endocytic pathways of human adenovirus (Ad) types 2 and 5.
  • To understand how Ad uptake influences cellular processes and infection.

Main Methods:

  • Survey of Ad uptake mechanisms in relation to cellular endocytosis.
  • Analysis of Ad2/Ad5 interaction with coxsackie virus B Ad receptor (CAR) and integrin coreceptors.
  • Investigation of clathrin-mediated endocytosis and macropinocytosis involvement.

Main Results:

  • Ad2/Ad5 uptake in epithelial cells is primarily clathrin-mediated, involving dynamin and AP2.
  • Macropinocytosis is simultaneously induced but not essential for epithelial cell entry.
  • Macropinocytosis becomes a productive entry pathway when Ad is targeted to Fcgamma receptor CD64 on hematopoietic cells.
  • Adenovirus modulates endocytic trafficking and disrupts cellular compartments, releasing contents to the cytosol.

Conclusions:

  • Adenovirus entry is a complex process involving multiple endocytic pathways.
  • The specific entry route dictates infection efficiency and cellular targeting.
  • Understanding these pathways allows for the development of targeted Ad vectors for therapeutic applications.

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