Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

A method of patch clamp recording in hyperbaric oxygen.

C V Jamieson1, A G MacDonald

  • 1Department of Biomedical Sciences, University of Aberdeen, Aberdeen AB24 2TZ, Scotland, UK.

Undersea & Hyperbaric Medicine : Journal of the Undersea and Hyperbaric Medical Society, Inc
|June 12, 2003
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Triploidy induction in recently fertilized Atlantic salmon ova using anaesthetics.

Aquaculture (Amsterdam, Netherlands)·2018
Same author

Constitutional symptoms at the onset of rheumatoid arthritis and subsequent arterial stiffness.

Clinical rheumatology·2010
Same author

SILICONES AND BLOOD COAGULATION.

Canadian Medical Association journal·2010
Same author

Malaria Problems Pertinent to Prevention.

Proceedings of the Royal Society of Medicine·2009
Same author

Effect of high hydrostatic pressure on the bacterial mechanosensitive channel MscS.

European biophysics journal : EBJ·2005
Same author

Effects of gravity and hydrostatic pressure on angular acceleration coding sensory neurones in the crab and dogfish.

Journal of gravitational physiology : a journal of the International Society for Gravitational Physiology·2004
Same journal

Side Effects of Hyperbaric Oxygen Therapy.

Undersea & hyperbaric medicine : journal of the Undersea and Hyperbaric Medical Society, Inc·2026
Same journal

Avascular Necrosis (Aseptic Osteonecrosis).

Undersea & hyperbaric medicine : journal of the Undersea and Hyperbaric Medical Society, Inc·2026
Same journal

Adjunctive Hyperbaric Oxygen in the Treatment of Thermal Burns.

Undersea & hyperbaric medicine : journal of the Undersea and Hyperbaric Medical Society, Inc·2026
Same journal

Principles for the Design, Validation, and Acceptance of Decompression Procedures.

Undersea & hyperbaric medicine : journal of the Undersea and Hyperbaric Medical Society, Inc·2026
Same journal

A Non-Invasive Gas Exchange Monitor To Assess Swimming-Induced Pulmonary Edema.

Undersea & hyperbaric medicine : journal of the Undersea and Hyperbaric Medical Society, Inc·2026
Same journal

Dive Injury and Jellyfish Sting Case Study.

Undersea & hyperbaric medicine : journal of the Undersea and Hyperbaric Medical Society, Inc·2026
See all related articles

This study introduces a novel patch clamp method for hyperbaric oxygen experiments. Hyperbaric oxygen increased ionic permeability of excised patches, affecting BK channel activity.

Area of Science:

  • Biophysics
  • Cell Biology
  • Physiology

Background:

  • Patch clamp electrophysiology is crucial for studying ion channel function.
  • Hyperbaric oxygen (HBO) is used therapeutically, but its cellular effects, particularly on ion channels, are not fully understood.
  • High hydrostatic pressure techniques offer insights into cellular responses under extreme conditions.

Purpose of the Study:

  • To develop and validate a patch clamp recording method suitable for hyperbaric oxygen (HBO) environments.
  • To investigate the effects of HBO on the ionic permeability and BK channel activity in cell membrane patches.

Main Methods:

  • Adaptation of high hydrostatic pressure patch clamp techniques for hyperbaric oxygen conditions.
  • Recording of excised membrane patches from HEK 293 cells expressing BK channels under HBO.

Related Experiment Videos

  • Analysis of ionic permeability, seal resistance, and BK channel activity using electrophysiological measurements.

    • Main Results:

    • Excised patches rapidly equilibrated with hyperbaric oxygen within 10 minutes.
    • 1 MPa of oxygen significantly increased patch ionic permeability, an effect potentiated by ferrous ions.
    • Seal resistance remained unaffected, but small negative currents reduced signal-to-noise ratio, impacting BK channel activity observation.

    Conclusions:

    • The developed patch clamp method allows for studying cellular responses under hyperbaric oxygen.
    • Hyperbaric oxygen exposure alters membrane properties and ion channel function, specifically affecting BK channels.
    • Further research is needed to elucidate the mechanisms behind HBO-induced changes in ion channel activity and membrane permeability.