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Updated: Sep 25, 2026

Extraction and Detection of Geosmin and 2-Methylisoborneol in Water and Fish using High-Capacity Sorptive Extraction Probes and GC-MS
Published on: July 3, 2025
High-affinity monoclonal antibodies for detection of the microbial metabolite, 2-methylisoborneol
Leslie C Plhak1, Eun Sung Park
1Department of Food Science, Louisiana Agricultural Experiment Station and Louisiana State University, Baton Rouge 70803, USA. lcplhak@facstaff.wisc.edu
Abstract:
The production of 2-methylisoborneol (MIB) by certain fungi and algae can contribute musty off-flavors to foods and water supplies if uncontrolled. The goal of this research was to develop a nonsensory simple method for the detection of MIB. Anti-MIB monoclonal antibodies were produced by immunizing mice with borneol-conjugated protein and selecting positive clones with an MIB-protein conjugate. An indirect competitive immunoassay developed using this antibody had a detection limit of 0.6 microg L(-)(1) and an I(50) value of 5 microg L(-)(1). Detection was relatively specific for MIB and showed 20% cross-reactivity with borneol or isoborneol and 4-5% cross-reactivity with camphor. No cross-reactivity to geosmin was observed.
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