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Clonal diversity of Shiga toxin-producing Escherichia coli O103:H2/H(-) in Germany
Rita Prager1, Almut Liesegang, W Voigt
1National Reference Centre of Salmonella and other enterics, Robert Koch Institute, Bereich Wernigerode, Burgstr. 37, D-38855 Wernigerode, Germany.
Abstract:
Shiga toxin producing Escherichia coli O103:H2/H(-) belong to the third most frequently isolated EHEC serotypes in Germany following isolates of O157:H7/H(-) and O26:H11/H(-). A total of 145 respective E. coli 103 isolates from single cases of diarrhoea and haemolytic uremic syndrome (HUS) in 1997-2000 were characterised by a range of molecular subtyping methods (PFGE, P-gene profiling, ribotyping, electrotyping) and phage typing in order to analyse their genetic relatedness and the practicability for new epidemiological tracing back. All isolates cluster into a distinct EHEC subgroup and reveal a high clonal diversity together with a considerable stability. Since strains of this serotype rank up to the third most frequently isolated EHEC in Germany a large population of this serotype, and therefore, a great supply of such strains may exist in this country.
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