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Related Experiment Videos

Bone induction by BMP-2 transduced stem cells derived from human fat.

Jason L Dragoo1, Joon Y Choi, Jay R Lieberman

  • 1Laboratory for Regenerative Bioengineering and Repair, Department of Surgery, UCLA School of Medicine, 90095-6092, USA.

Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society
|June 12, 2003
PubMed
Summary

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Processed lipoaspirate cells (PLAs) show significant osteogenic potential, rapidly differentiating into bone precursors. These mesenchymal stem cells, when genetically modified with BMP-2, outperform bone marrow aspirate cells in bone formation for tissue engineering.

Area of Science:

  • Stem Cell Biology
  • Regenerative Medicine
  • Gene Therapy

Background:

  • Pluripotent mesenchymal progenitor cells are crucial for bone regeneration.
  • Liposuction aspirates offer a rich source of these cells (processed lipoaspirate cells or PLAs).
  • Bone morphogenetic protein-2 (BMP-2) is a key factor in osteogenesis.

Purpose of the Study:

  • To evaluate the osteogenic potential of PLAs compared to bone marrow aspirate cells (BMAs).
  • To investigate the effects of recombinant human BMP-2 (rh-BMP-2) and adenovirus-mediated BMP-2 gene delivery (Ad-BMP-2) on PLA and BMA osteogenesis.

Main Methods:

  • PLAs were isolated from liposuction aspirates via proteolytic digestion.
  • Cells were exposed to rh-BMP-2 or Ad-BMP-2 for 4 or 7 days.

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  • Osteogenic potential was assessed using histochemistry, spectrophotometry, RT-PCR, Western blotting, and ELISA. In vivo studies involved implanting PLA-Ad-BMP-2 cells on matrices in SCID mice.

    • Main Results:

    • PLAs treated with rh-BMP-2 or Ad-BMP-2 demonstrated significantly higher bone precursor production than osteoblasts and BMAs.
    • Successful BMP-2 gene transduction and expression were confirmed in both cell types.
    • In vivo implantation of PLA-Ad-BMP-2 cells resulted in confirmed bone formation within six weeks.

    Conclusions:

    • PLAs can be effectively transfected with the BMP-2 gene, rapidly inducing an osteoblast phenotype.
    • Transduced PLAs exhibit superior bone precursor generation and faster matrix calcification compared to transduced BMAs.
    • PLAs represent a promising cell source for gene therapy and tissue engineering applications in bone regeneration.