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m-CPP-induced self-grooming is mediated by 5-HT2C receptors
Marton Graf1, Sandor Kantor, Zsuzsanna E Anheuer
1Laboratory of Neurochemistry and Experimental Medicine, National Institute of Psychiatry and Neurology, Huvosvolgyi ut 116, Budapest H-1021, Hungary.
Behavioural Brain Research
|June 12, 2003
Summary
m-Chlorophenylpiperazine (m-CPP) triggers self-grooming in rats by activating 5-HT(2C) receptors. Depleting brain serotonin enhances this effect, indicating receptor sensitization.
Area of Science:
- Neuroscience
- Pharmacology
Background:
- m-Chlorophenylpiperazine (m-CPP) is a 5-HT receptor agonist known to induce self-grooming in rats.
- m-CPP can also worsen symptoms in individuals with obsessive-compulsive disorder (OCD).
Purpose of the Study:
- To investigate the specific roles of 5-HT(2B) and 5-HT(2C) receptors in m-CPP-induced self-grooming.
- To understand how serotonin depletion affects m-CPP's behavioral impact.
Main Methods:
- Administered m-CPP to male Sprague-Dawley rats to measure self-grooming behavior.
- Utilized subtype-selective antagonists SB-242084 (5-HT(2C)) and SB-215505 (5-HT(2B)).
- Depleted brain serotonin using p-chlorophenylalanine (p-CPA) before m-CPP administration.
Main Results:
- m-CPP significantly increased self-grooming in a bell-shaped dose-response pattern, peaking at 0.6 mg/kg.
- SB-242084 (5-HT(2C) antagonist) effectively reversed m-CPP-induced self-grooming.
- SB-215505 (5-HT(2B) antagonist) did not block the m-CPP effect.
- Serotonin depletion via p-CPA significantly enhanced m-CPP-induced responses.
Conclusions:
- Direct activation of 5-HT(2C) receptors mediates m-CPP-induced self-grooming.
- Depletion of brain serotonin sensitizes 5-HT(2C) receptors, amplifying the behavioral effects of m-CPP.