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Related Experiment Videos

Darting behavior: a quantitative movement pattern designed for discrimination and replicability in mouse locomotor

Neri Kafkafi1, Michal Pagis, Dina Lipkind

  • 1National Institute on Drug Abuse, National Institutes of Health, 5500 Nathan Shock Drive, The Johns Hopkins Bayview Medical Center, Bldg. C, Baltimore, MD 21224, USA. nkafkaki@intra.nida.nih.gov

Behavioural Brain Research
|June 12, 2003
PubMed
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Software for Exploring Exploration (SEE) enables precise measurement of rodent open-field behavior, like darting. This method reliably distinguishes mouse strains and drug effects across labs.

Area of Science:

  • Neuroscience
  • Behavioral Science
  • Pharmacology

Background:

  • Rodent open-field behavior is crucial for studying neurological and psychiatric conditions.
  • Existing behavioral measures often lack high genotype discrimination and cross-laboratory replicability.
  • Software for Exploring Exploration (SEE) offers a novel approach to behavioral endpoint design.

Purpose of the Study:

  • To develop and validate a novel behavioral endpoint, "darting," using SEE.
  • To assess the genotype discrimination and cross-laboratory replicability of the darting measure.
  • To investigate the effects of cocaine and amphetamine on darting behavior in different mouse strains.

Main Methods:

  • Utilized Software for Exploring Exploration (SEE) to analyze open-field behavior in C57BL/6J (B6) and DBA/2J (D2) mice.

Related Experiment Videos

  • Collected data across three independent laboratories.
  • Administered cocaine and amphetamine to assess drug effects on darting behavior.
  • Defined "darting" as high acceleration during movement and reduced movement during stops.

    • Main Results:

    • DBA/2J mice exhibited significantly more darting than C57BL/6J mice across all laboratories, despite lower overall activity.
    • Darting behavior differences between strains were robust to cocaine administration (up to 20mg/kg).
    • Amphetamine (up to 5mg/kg) slightly altered darting behavior despite significantly increasing overall activity.
    • The darting measure demonstrated high replicability in independent experiments.

    Conclusions:

    • The SEE-developed "darting" measure effectively discriminates between mouse genotypes and is replicable across laboratories.
    • Darting behavior provides a sensitive endpoint for assessing stimulant drug effects in rodents.
    • This strategy can be applied to develop further discriminative and replicable open-field behavioral endpoints.