Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Porcine Ig isotypes: function and molecular characteristics.

A Crawley1, B N Wilkie

  • 1Department of Pathobiology, University of Guelph, Guelph, Ont., Canada N1G 2W1. acrawley@uoguelph.ca

Vaccine
|June 12, 2003
PubMed
Summary

Porcine immunoglobulin (Ig) isotypes reflect immune responses. This study found porcine IgG(2) activates complement better than IgG(1), suggesting functional differences in disease resistance and susceptibility.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Proton beam therapy: neuroradiology considerations and challenges.

Clinical radiology·2026
Same author

Improved White Matter Cerebrovascular Reactivity after Revascularization in Patients with Steno-Occlusive Disease.

AJNR. American journal of neuroradiology·2018
Same author

A Novel MRI Biomarker of Spinal Cord White Matter Injury: T2*-Weighted White Matter to Gray Matter Signal Intensity Ratio.

AJNR. American journal of neuroradiology·2017
Same author

Clinically Feasible Microstructural MRI to Quantify Cervical Spinal Cord Tissue Injury Using DTI, MT, and T2*-Weighted Imaging: Assessment of Normative Data and Reliability.

AJNR. American journal of neuroradiology·2017
Same author

Frontotemporal correlates of impulsivity and machine learning in retired professional athletes with a history of multiple concussions.

Brain structure & function·2015
Same author

Prophylaxis of experimentally induced ovomucoid allergy in neonatal pigs using Lactococcus lactis.

Veterinary immunology and immunopathology·2010

Area of Science:

  • Immunology
  • Veterinary Medicine
  • Molecular Biology

Background:

  • Antibody (immunoglobulin, Ig) isotypes are dictated by Type 1 (interferon-gamma [IFN-γ], interleukin-12 [IL-12]) and Type 2 (interleukin-4 [IL-4], interleukin-10 [IL-10]) cytokines, influencing immune response polarization.
  • In pigs, protection against Actinobacillus pleuropneumoniae correlates with a higher IgG(1):IgG(2) antibody ratio.
  • The functional differences between porcine Ig isotypes remain largely undescribed.

Purpose of the Study:

  • To investigate the functional differences between porcine immunoglobulin (Ig) isotypes, specifically IgM, IgG(1), and IgG(2).
  • To compare the complement (C') activating and opsonizing abilities of purified porcine IgG(1) and IgG(2) in vitro.
  • To analyze the amino acid sequences of porcine IgG isotypes for predicted complement-binding efficiencies.

Related Experiment Videos

Main Methods:

  • Affinity purification of porcine IgM, IgG(1), and IgG(2) specific for hen eggwhite lysozyme (HEWL).
  • In vitro assays to assess complement (C') activation and opsonization capabilities of purified Ig isotypes.
  • Amino acid sequence analysis of porcine IgG isotypes to predict functional differences in C' activation.

Main Results:

  • Porcine IgG(2) demonstrated superior complement (C') activating ability compared to IgG(1) in vitro.
  • Neither IgG(1) nor IgG(2) effectively opsonized HEWL-conjugated sheep erythrocytes in vitro.
  • Sequence analysis indicated that porcine IgG(2a), IgG(2b), and IgG(4) possess more flexible hinge regions, suggesting more efficient C' activation than IgG(1) and IgG(3).

Conclusions:

  • Porcine IgG isotypes exhibit distinct functional capabilities, with IgG(2) showing enhanced complement activation.
  • These functional differences in porcine IgG isotypes likely contribute to varying degrees of resistance and susceptibility to infections.
  • Understanding these isotype functions is crucial for developing effective strategies against swine diseases.