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Isolation of high molecular weight DNA from soil for cloning into BAC vectors
Andrew E Berry1, Claudia Chiocchini, Tina Selby
1Department of Biological Sciences, University of Warwick, Gibbet Hill Rd, Coventry CV4 7AL, UK. aberry@bio.warwick.ac.uk
FEMS Microbiology Letters
|June 12, 2003
Abstract:
Isolation of high molecular weight DNA fragments from soil, in excess of 1 Mb, and of sufficient quality for cloning into an Escherichia coli-streptomycete artificial chromosome vector is described. The combination of indirect extraction of cells, using a nycodenz extraction technique, followed by lysis of biomass immobilised in agarose plugs, allowed fragments in excess of 1 Mb to be purified.