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Myelin Oligodendrocyte Glycoprotein (MOG35-55) Induced Experimental Autoimmune Encephalomyelitis (EAE) in C57BL/6 Mice
Published on: April 15, 2014
Genetic variation in myelin oligodendrocyte glycoprotein expression and susceptibility to experimental autoimmune
Maria Pagany1, Maja Jagodic, Carole Bourquin
1Department of Neuroimmunology, Max-Planck Institute for Neurobiology, Am Klopferspitz 18a, 82152 Martinsried, Germany. pagany@neuro.mpg.de
Abstract:
Myelin oligodendrocyte glycoprotein (MOG) is encoded within the RT1.M region of the rat MHC a susceptibility locus for MOG-induced experimental autoimmune encephalomyelitis (EAE). We report that the enhanced susceptibility of Brown Norway (BN) rats to MOG-EAE is associated with higher expression of MOG mRNA and protein in the nervous system than in the less susceptible Lewis (LEW) strain. MOG mRNA was also detected in the immune system, but there was no correlation with disease susceptibility. These results suggest that differences in the expression of MOG in the target organ, rather than in the immune system may influence susceptibility to MOG-EAE.
Insights
Enhanced susceptibility to myelin oligodendrocyte glycoprotein (MOG) induced experimental autoimmune encephalomyelitis (EAE) in Brown Norway rats is linked to higher MOG expression in the nervous system. Immune system MOG levels did not correlate with disease susceptibility.
Area of Science:
- Neuroimmunology
- Autoimmunity
- Genetics
Background:
- Myelin oligodendrocyte glycoprotein (MOG) is a key autoantigen in experimental autoimmune encephalomyelitis (EAE).
- The Major Histocompatibility Complex (MHC) RT1.M region in rats is a known susceptibility locus for MOG-EAE.
- Differential susceptibility to MOG-EAE exists between rat strains, such as Brown Norway (BN) and Lewis (LEW).
Purpose of the Study:
- To investigate the role of MOG expression levels in the differential susceptibility of BN and LEW rats to MOG-EAE.
- To determine if MOG expression in the nervous system or immune system correlates with disease susceptibility.
Main Methods:
- Comparison of MOG mRNA and protein expression in the nervous system and immune cells of BN and LEW rats.
- Correlation analysis between MOG expression levels and experimental autoimmune encephalomyelitis (EAE) susceptibility.
Main Results:
- Brown Norway (BN) rats exhibited significantly higher MOG mRNA and protein expression in the nervous system compared to Lewis (LEW) rats.
- MOG mRNA was detected in the immune system of both rat strains.
- No correlation was found between MOG expression in the immune system and susceptibility to MOG-EAE.
Conclusions:
- Higher expression of MOG in the central nervous system is associated with enhanced susceptibility to MOG-induced EAE in BN rats.
- Differences in MOG expression within the target organ (nervous system) appear to be a critical factor influencing MOG-EAE susceptibility.
- Immune system MOG expression levels do not seem to be a primary determinant of susceptibility to MOG-EAE.
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