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Overcoming Unresponsiveness in Experimental Autoimmune Encephalomyelitis (EAE) Resistant Mouse Strains by Adoptive Transfer and Antigenic Challenge
Published on: April 9, 2012
Experimental autoimmune encephalomyelitis relapses are reduced in heterozygous golli MBP knockout mice
Rhonda R Voskuhl1, Thomas M Pribyl, Kathy Kampf
1Reed Neurological Research Center, UCLA Department of Neurology, 710 Westwood Plaza, Los Angeles, CA 90095, USA. rvoskuhl@ucla.edu
Abstract:
Increased golli MBP (golli) expression has been observed in the peripheral immune system of mice in the relapsing phase of EAE, raising the possibility that golli MBP expression in the periphery may contribute to relapses. Here we describe the generation of golli MBP-deficient mice and a comparison of the clinical course of EAE between heterozygous (golli(+/-)) and wild-type (golli(+/+)) mice. There was no difference between the two groups in incidence of disease, severity of the first episode of disease, or remission after the first episode. However, there was a significant reduction in relapses in golli(+/-) mice vs. controls, suggesting a role for golli proteins in the relapses in EAE.
Insights
Golli MBP deficiency in mice reduced relapses in experimental autoimmune encephalomyelitis (EAE). This suggests golli proteins play a role in EAE relapses, impacting potential therapeutic strategies.
Area of Science:
- Neuroimmunology
- Autoimmune Diseases
- Molecular Biology
Background:
- Increased golli MBP expression is noted in the peripheral immune system during the relapsing phase of EAE.
- This observation suggests a potential role for peripheral golli MBP in exacerbating EAE relapses.
Purpose of the Study:
- To investigate the role of golli MBP in EAE by generating golli MBP-deficient mice.
- To compare the clinical course of EAE in heterozygous golli(+/-) mice and wild-type (golli(+/+)) mice.
Main Methods:
- Generation of golli MBP-deficient mice.
- Induction of experimental autoimmune encephalomyelitis (EAE) in heterozygous and wild-type mice.
- Clinical assessment of EAE incidence, severity, remission, and relapse rates.
Main Results:
- No significant differences were observed in disease incidence, initial severity, or first episode remission between golli(+/-) and golli(+/+) mice.
- A significant reduction in the frequency of relapses was observed in golli(+/-) mice compared to controls.
Conclusions:
- Golli MBP deficiency does not affect the initial development or severity of EAE.
- Golli proteins appear to play a crucial role in the relapse phase of EAE, indicating a potential therapeutic target.
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