Related Experiment Videos
Chick receptor tyrosine phosphatase Psi is dynamically expressed during somitogenesis
Birgit Aerne1, Andrew Stoker, David Ish-Horowicz
1Developmental Genetics Laboratory, Cancer Research UK, Lincoln's Inn Fields, WC2A 3PX London, UK. birgit.aerne@cancer.org.uk
Gene Expression Patterns : GEP
|June 12, 2003
Summary
Researchers cloned chick receptor tyrosine phosphatase Psi (cRPTPPsi) to study its role in vertebrate development. This phosphatase is dynamically expressed during somitogenesis and in various developing organs.
Area of Science:
- Developmental Biology
- Molecular Biology
- Genetics
Background:
- Receptor tyrosine phosphatases (RPTPs) are crucial for cell signaling.
- Their roles in vertebrate development, especially somitogenesis, require further elucidation.
- Chick receptor tyrosine phosphatase Psi (cRPTPPsi) is a potential key player.
Purpose of the Study:
- To clone chick receptor tyrosine phosphatase Psi (cRPTPPsi).
- To investigate the expression patterns of cRPTPPsi during vertebrate development.
- To understand the role of cRPTPPsi in somitogenesis.
Main Methods:
- Gene cloning of chick receptor tyrosine phosphatase Psi (cRPTPPsi).
- Analysis of cRPTPPsi expression via in situ hybridization or similar techniques.
- Correlation of expression patterns with developmental processes like somitogenesis.
Main Results:
- cRPTPPsi was successfully cloned from chick embryos.
- Dynamic expression of cRPTPPsi observed in somites and presomitic mesoderm.
- Expression restricted to dermomyotome in differentiating somites.
- Additional expression in intermediate mesoderm, neurogenic/sensory organs, limb bud, and heart.
Conclusions:
- cRPTPPsi exhibits dynamic and specific expression patterns during vertebrate development.
- Its expression correlates with key developmental events, particularly somitogenesis.
- cRPTPPsi likely plays a significant role in the development of multiple organ systems.