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Related Experiment Videos

Pluripotent stem cells identified in multiple murine tissues.

Jonathan C Howell1, Wei-Hua Lee, Paul Morrison

  • 1Division of Hematology/Oncology and Indiana Elks Cancer Research Center, Department of Medicine, Indiana University School of Medicine, Indianapolis, Indiana 46202, USA.

Annals of the New York Academy of Sciences
|June 12, 2003
PubMed
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Researchers identified a rare, common population of pluripotent stem cells (PSCs) in multiple mouse tissues. These PSCs demonstrated broad differentiation potential, including transdifferentiation into various cell types, suggesting a universal stem cell.

Area of Science:

  • Stem Cell Biology
  • Developmental Biology
  • Regenerative Medicine

Background:

  • Pluripotential stem cells (PSCs) have been reported in various tissues, but their exact nature and definition remain unclear.
  • The potential for a common, rare PSC population with broad differentiation capabilities across different tissues is hypothesized.

Purpose of the Study:

  • To identify and characterize a common, rare population of PSCs in murine tissues.
  • To investigate the transdifferentiation potential of these PSCs in response to diverse microenvironmental cues.
  • To determine if these cells represent a universal pluripotent stem cell.

Main Methods:

  • Isolation and culture of phenotypically defined CD45(-)Sca-1(+)c-kit(-) cells from murine skeletal muscle.
  • Culture of cells under conditions promoting hematopoietic, myogenic, neuronal, and adipocytic differentiation.

Related Experiment Videos

  • Intracerebellar transplantation of cells into Purkinje cell degenerative (pcd) mice.
  • Phenotypic analysis of cell suspensions from various murine tissues (brain, blood, intestinal epithelium).

    • Main Results:

    • Muscle-derived CD45(-)Sca-1(+)c-kit(-) cells differentiated into hematopoietic cells and cells expressing myogenic, neuronal, and adipocytic markers.
    • Transplantation into pcd mice improved behavior, suggesting functional neuronal cell contribution.
    • CD45(-)Sca-1(+)c-kit(-) cells were identified in brain, blood, and intestinal epithelium.

    Conclusions:

    • A phenotypically common, multipotent cell (CD45(-)Sca-1(+)c-kit(-)) can be identified across multiple murine tissues.
    • This cell exhibits broad differentiation and transdifferentiation potential.
    • These findings suggest the existence of a universal pluripotent stem cell residing in various tissues.