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Analysis of food colorants by capillary electrophoresis with large-volume sample stacking
Hsi-Ya Huang1, Chen-Wen Chiu, Show-Li Sue
1Department of Chemistry, Center for Nanotechnology, Chung Yuan Christian University, Chung-Li 320, Taiwan. hyhuang@cycu.edu.tw
Abstract:
A technique combining an on-capillary concentration method known as large-volume sample stacking and high-efficiency CE separation has been developed to analyze and detect colorants in several food samples, such as soft drinks, jellies and milk beverages. Following optimization, this technique significantly reduced the limits of detection of eight food colorants commonly used in food products by up to two orders of magnitude when compared with the conventional capillary electrophoresis method. The developed technique was able to successfully determine colorants in food samples that had concentrations as low as 0.1-0.5 microg/ml.