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An effective sporicidal reagent against Bacillus subtilis spores
Nori Kida1, Yasushi Mochizuki, Fumiaki Taguchi
1Niigata Quarantine Station, Ministry of Health, Labour and Welfare, 1-5-4, Ryugashima, Niigata, Niigata 950-0072, Japan. n-kida@forth.go.jp
Abstract:
We developed a reagent which showed significant sporicidal activity against Bacillus subtilis spores. This reagent was composed of ethylenediaminetetraacetic acid, disodium salt (EDTA-2Na), ferric chloride hexahydrate (FeCl3 x 6H2O) and ethanol (tentatively designated as the ethanol reagent). The ethanol reagent showed pH- and temperature-dependent sporicidal activity. At pH 0.3, its activity was almost the same as that of 0.05% sodium hypochlorite at 20 C and was higher at 37 C than at 20 C. The activity of the ethanol reagent was similar both with and without 10% serum. The ethanol reagent might be applicable for disinfecting Bacillus spores.